Polysaccharides-based nanoparticles as drug delivery systems. Molecular weight (M v) and hydrodynamic properties can be calculated from there. At 25C both values of a and kM-H are within the range reported by [3, 8, 16]. a Microscopy images of micropore and b quantified data using ImageJ after 0 (immediately), 4, 8, and 24h according to the number of times microproration was done (n=150, *: p<0.05). SEC of mono-carboxymethyl cellulose (CMC) in a wide range of pH, MarkHouwink constants. These constants can be determined experimentally by measuring the intrinsic viscosity of several polymer samples for which the molecular weight has been determined by an independent method (i.e. Drug Deliv Syst. GYA primarily conducted research. Differences were considered statistically significant at p <0.05. Global hydrodynamic analysis of the molecular flexibility of galactomannans. Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. ; Yevlampieva N.P. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). 1905/05) for their financial support. In this study, we investigated the effectiveness of RF microporation technology in vitro, by evaluating the permeation of fluorescent-conjugated dextran (FITC-dextran) of various molecular weights through the human and pig skin after microporation, using a transdermal diffusion cell system. 20K groups (n=3, *: p<0.05, **: p<0.01). Hennink, W.E. He, L.; Niemeyer, B. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). TEWL measurements were taken 30min after microporation of the human and pig skin. In this work, hydrodynamic properties are investigated in different dextran aqueous solutions with different molecular weights (8.8-2000kDa), in a temperature range from 20C to 50C. WebThis value is sensible given that i j = 0 and that the search radius of the clustering algorithm is slightly larger than the average segment length of our Dextran model (b 5.5 nm). @ Nilimesh See attached. Ed., 9(2), 85-90. We believe that the microporation device can be used as a potential technique for effective transdermal drug delivery. Carbohydrate Polymers, 68, 477-488. Dextrans in Aqueous Solution. The results are shown to be in good agreement with available hydrodynamic models for spherical solutes in cylindrical pores with the effective solute to pore size ratio evaluated using a partitioning model which implicitly The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Ortega, A.; Garca de la Torre, J. High Manufacturing Standards of Molecular Probes Dextrans. WebFITC Products. The https:// ensures that you are connecting to the Anal. The first term on the left-hand side of equation 2 (i/c) has a linear relation as a function of the concentration (c in g/cm3), where is the intrinsic viscosity [] is given by (c0) [42, 43]. Web54 we estimated that the hydrodynamic radii of 3-5 kDa dextran, 20 kDa dextran, and 70 kDa dextran are 2 55 nm, 5 nm, and 6 nm, respectively. 2018;1:10915. The Mark-Houwink a constant is close to 0.75 (or higher) for these good solvents. The intrinsic viscosity classical theory of random coils at conditions []0 predicts the quotient a0 = d ln[]0/d ln M to decrease from unity to 0.5 as the chain length increases. Hydrodynamic cavitation (HC), a common phenomenon that can produce local excessive temperature, high pressure and large amount of energy. Huggins equation [41] gives the relation to the relative viscosity increase (i). For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. For data requests, please contact the authors. The term f/f0 is sometimes denoted as P, Perrin constant. Drug Deliv. Hi Khanh, Alan is taking a little time off of Research Gate at the moment, but here is some information in reply to your question. Banga AK. Doke SK, Dhawale SC. 2014 Sep;5(9):2324-30. doi: 10.1039/c4fo00319e. Salunkhe SS, Bhatia NM, Pokharkar VB, Thorat JD, Bhatia MS. Topical delivery of idebenone using nanostructured lipid carriers: evaluations of sun-protection and anti-oxidant effects. (2001). Learn More. Kirkland, J. J.; Dilks, C. H.; Rementer, S. W. (1992). Guner, Ali (1999). Ubbelohde, Leo. (2008). Advanced Drug Delivery Reviews, 60, 1650-1662. The permeability of fluorescein isothiocyanate-dextrans (FITC-dextrans) with different molecular weights through the pig and human cadaver skins were measured using Franz diffusion cell. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Optical properties of dextran in solution and in films. ; Fran, H.P. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. J Pharm Sci. The 70-kDa FITC dextran reached the jejunum after 15 min and passed the entire small intestine within 1 h after its administration, demonstrating that 4-kDa FITC dextran measured in plasma 1 h after its oral application is a marker of small intestinal permeability. Senti, F. R.; Hellman, N. N.; Ludwig, G.; Babcock, E.; Tobin, R.; Class, C. A., (1955). Transdermal drug delivery: overcoming the skins barrier function. Int J Food Sci Nutr. The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Mark-Houwink values confirm that for these conditions dextrans behave as rigid spheres with a tendency at compaction as temperature increases. (1999). DOI: 10.12691/jpbpc-1-1-3, Received September 18, 2013; Revised October 23, 2013; Accepted November 22, 2013. We observe that this maximum radius is below Springer Nature. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was The exponent a is a function of polymer geometry, and varies from 0.5 to 2 and exponent is dimensionless. HSE and DK helped to porate the skin and to measure TEWL. 2012;29(7):201729. WebLabeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the Proc. Harding, Stephen E. (2005). Biomater Res 24, 22 (2020). Gums and stabilisers for the Food Industry, 5, 447-450. Molecular weight determination of polysaccharides. 2009;301(8):60913. Bahary, W. S.; Hogan, M. P.; Jilani, M.; Aronson, M. P. (1995). Macromolecules. Rotureau, E.; Dellacherie, E.; Durand, A. (2008). Epub 2021 Feb 9. Die Makromolekulare Chemie, 148(1), 229-238. There is a deviation from linearity after 580kDa [69, 70]. The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. Webof overall macromolecular geometry and hydrodynamic draining. The formula is verified for different systems of polymer-solvent and the values are in accord with those obtained by extrapolation. European Polymer Journal, 42, 1086-1092. Kasaai, M. R.; Arul, J.; Charlet, G. (2000). One day before the experiment, the skin was stabilized in the skin culture medium. 4h after microporation, the sizes of the micropore decreased slightly. Polymer molecular weight distribution. The human and pig skin permeability of FITC-dextran (M.W. Guner, A.; Kibarer, G. (2001). (1954). Carbohydrate Polymers 99, 199-207. Pharmaceutical Quality Dextran 40 is available from Pharmacosmos as active pharmaceutical ingredients (API) as excipient for final formulation, drug product, for medical devices, for production of solutions for injection and infusion and much more. Drug Discov Today Technol. Biomaterials. If there are particles in the sample, the incident laser light gets scattered in all directions. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Smith, David R.; Raymonda, John W. (1972). Carbohydrate Polymers, 39, 377-380. Webdextran hydrodynamic radius. Results and Discussion The microscopic dynamics of proteins are probed in a light scattering experiment. The column was eluted with bidestilled water a flow rate of 0.8 ml/min, injection 5L, back pressure 200psi. The Mark-Houwink parameters indicate the dependence with temperature (T) in the range from 20 to 50C, ie with increasing T a increases and kM-H decreases. 2016;9706:97061E6. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. Unperturbed dimensions and the theta temperature of dextran in ethylene glycol solutions. Challenges for the modern analytical ultracentrifuge analysis of polysaccharides. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. (1937). These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Figure 3 shows that the parameters vary with temperature, evidencing a clear functionality of them. Because of the recent animal protection rules which have restricted animal experiments [19], we used human cadaver skin as an alternative to animals. 2006;3(3):37385. Kano S, Todo H, Furui K, Kenichi S, Tokudome Y, Hashimoto F, Kojima H, Sugibayashi K. Comparison of several reconstructed cultured human skin models by microscopic observation: their usefulness as an alternative membrane for skin in drug permeation experiments. Progress in Polymer Science, 30, 38-70. Dextran theta temperature between 43.14 and 44.44C for all the range of molecular weights explains the deviation at 45 and 50C [36, 37]. Catiker, E.; Guner, A. Would you like email updates of new search results? H&E stained images were analyzed using Image J software. 10% neutral buffered formalin was purchased from Hisko (Gunpo, Korea). Ghimici, Luminita; Nichifora, Marieta; Eich, Andreas; Wolf, Bernhard A. The most widely applied coatings for FDA-approved SPIONs are dextran and carboxydextran. Kloster, C.; Bica C.; Rochas C.; Samios, D.; Geissler, E. (2000). A lot of research has been devoted to dextrans modification in order to describe special characteristics relevant to specific applications such as surfactants [16], as visible marker [17], polysaccharides-based nanoparticles, covalent crosslinking [18], ionic crosslinking, polyelectrolyte complex, and the self-assembly of hydrophobically modified polysaccharides, and hydrophilic modified polysaccharides [19]. The Mark-Houwink exponent bears the signature of a three-dimensional configuration of a polymer chain in the solvent environment. Clipboard, Search History, and several other advanced features are temporarily unavailable. 1994, 95-0355. Solvent effects on polysaccharide conformation. TEWL values before and immediately after microporation on human cadaver skin according to the number of times microproration was done (n=3). WebTry out PMC Labs and tell us what you think. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Solution properties of branched dextran. The Mark-Houwink a constant is close to 0.5 in poor solvents. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. WebTo address this perception, in the current study, FITC-dextran of different MWs (10, 70 and 150 kDa) was used to model the transdermal delivery of low MW biotherapeutics and mAbs with MWs of approximately 150 kDa. Soc., 16, 3A, 426-433. Fixable: Fixable via Lysine. Structure Properties of Dextran. Format), Citation-(BibTeX Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. 4K and M.W. WebTry out PMC Labs and tell us what you think. The solution viscosity () is related to the solvent viscosity (o), resulting in the relative viscosity. official website and that any information you provide is encrypted Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. Pharm Res. Keywords: Pure Appl. In Vitro Evaluation of Postbiotics Produced from Bacterial Isolates Obtained from Rainbow Trout and Nile Tilapia against the Pathogens, Dextran-Mimetic Quantum Dots for Multimodal Macrophage Imaging, Isolation of lactic acid bacteria starters from, Characterization of Antibacterial Cell-Free Supernatant from Oral Care Probiotic. Quintanilla-Pineda M, Achou CG, Daz J, Gutirrez-Falcon A, Bravo M, Herrera-Muoz JI, Pea-Navarro N, Alvarado C, Ibaez FC, Marzo F. Foods. All branched polysaccharide types could be described approximately by the hyperbranching theory. International Journal of Biological Macromolecules, 23, 135-141. (2013). The micropores were developed on pig skin and human cadaver skin with dermal and epidermal layers by the microporation device. Dextran is soluble in water, methyl sulphoxide, formamide, ethylene glycol, glycerol, 4-methylmorpholine-4-oxide, and hexamethyl phosphamide [4]. Cookies policy. Curley SA. 43-1, 115-147. Figure1 shows a schematic illustration of transdermal delivery after the application of the microporation device. Eng. Static and Dynamic Light-Scattering Solution Behavior of Pullulan and Dextran in Comparison. Ultimately they concluded that the temperature increment found to be an effective force in disturbing these interactions, mainly between polymer-polymer and polymer-solvent molecules for the used system. Formation of dextran hydrogels by crystallization. American Journal of Food Science and Technology 2013, 1(3), 60-66. ; Rjumtsev, E.I. WebThe micelles produced at pH 4.6 have a spherical shape and their size is dependent on the Maillard reaction: reaction time, molar ratio of casein to dextran, and molecular weight of dextran used. Barshtein, G.; Tamir, I.; Yedgar, S. (1998). 2010;4(1):117. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Lucifer yellow (LY) and FITC-labelled dextran stock solutions were prepared in Ringers-HEPES buffer (150 mM NaCl, 3.4 mM CaCl 2, 1.2 mM MgCl 2, 5.2 mM KCl, 0.5 mM NaHCO 3, 2.8 mM glucose, and 10 mM HEPES) and frozen at 20C. 2018 Jan;184(1):1-11. doi: 10.1007/s12010-017-2522-4. Materiaux 1-5. Rissmann R, Oudshoorn MHM, Hennink WE, Ponec M, Bouwstra JA. The Transdermal diffusion cell system was protected from light. ; Rani, K.Y. As a consequence of the diversity in their origin, the set of classical universal size independent quantities suffers from shortcomings. Dynamic light scattering study confirmed the mono-disperse nature of dextran with hydrodynamic radius of 900 nm. The genus Weissella: taxonomy, ecology and biotechnological potential. European Polymer Journal, 36, 2143-2146. Middelkoop E, Bogaerdt AJ, Lamme EN, Hoekstra MJ, Brandsma K, Ulrich MMW. Bookshelf Measurements were made with a Ubbelohde C type suspended level viscometer [57] (IVA, Argentina); with a diameter capillary of 0.81mm and water draining time of 35.89s at 20C, with viscometer constant of 0.027883 mm2/s [58]. WebThe Stokes radius or StokesEinstein radius of a solute is the radius of a hard sphere that diffuses at the same rate as that solute. Although we didnt conduct assay for inflammation, we could not find any inflammation symptoms such as redness, blisters, cracked, and thickening. Walkenhorst, S.; Olivier, R. (2003). In vitro studies with human cadaver skin is a viable, ethical, and economical alternative for in vivo animal studies. Progress in Measurement, Interpretation and Application to Structure in Dilute Solution. Figure5 shows the cumulative amount of the FITC-dextran (M.W. This low polydispersity realizes that the Mark-Houwink equation is forced at a given Mw regardless of the hyperbranched nature of the dextran molecule. WebAccording to the above theoretical analysis for the micro-roughness contact model, the relationship between the hydrodynamic pressure (HP) and the contact pressure (CP) is shown in Fig. Here we have the hydrodynamic radius estimate for the 3kDa dextran and 1.5 kDa dextran by the Zetasizer calculator, thanks to Alan. Alan F Rawle, t Biophysical Journal, 87, 4259-4270. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. ; Bataille, B. With FITC-dextran 20K, cumulative permeation in the human skin was 2.530.48M, and 3.340.19M in the pig skin, which significantly lower than FITC-dextran 4K. Cumulative amount of FITC-dextran permeated after microporation on the human and pig skin for FITC-dextran M.W.. a M.W. WebDextran displays excellent biocompatibility and have been used clinically for over 50 years. By studying the molecular weights of various solutions of polymer, Solomon-Ciuta arrived at the formula which allowed the calculation of the intrinsic viscosity of polymer solutions by a single viscosity determination. Prausnitz MR, Langer R. Transdermal drug delivery. Google Scholar. Recent Pat Drug Deliv Formul. The skins were from the abdomen of a 39-year-old caucasian woman (BMI: 24). The measurements of the transepidermal water loss (TEWL) were taken using the Tewameter (Courage-Khazaka Electronic GmbH, Cologne, Germany) to evaluate the skin barrier function after microporation. (2003). The receptor phase was collected immediately (time 0) and 2, 4, 8, 12, and 24h. This receptor phase solution was transferred to a 96-well plate and the skin-permeated FITC-dextran was measured using a microplate reader (Molecular Devices, Co. Ltd., Sunnyvale, CA, USA) at 405nm. Naik A, Kalia YN, Guy RH. Dextran. WebFluorescein isothiocyanatedextran average mol wt 60,000-76,000; CAS Number: 60842-46-8; Synonyms: FITCDextran; find Sigma-Aldrich-FD70S MSDS, related peer-reviewed papers, technical documents, similar products & more at Sigma-Aldrich The Viscosity of Dilute Solutions of Long-Chain Molecules. Schematic illustration of transdermal delivery after the application of the microporation device. Intrinsic viscosities of polyelectrolytes in the absence and in the presence of extra salt: Consequences of the stepwise conversion of dextran into a polycation. Keywords: intrinsic viscosity, dextrans, Mark-Houwink parameters, hydrodynamic, temperature, Journal of Polymer and Biopolymer Physics Chemistry, 2013 1 (1), 2013;3(5):3588605. Hi Sir Alexander Malm , Thank you very much! Really appreciate your help! Biomacromolecules 8, 2464-2475. Each skin pores was captured using a microscope and the obtained images were analyzed using ImageJ software (National Institutes of Health, Bethesda, USA). WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Journal of Applied Polymer Science, 27, 795-810. The mostly used commercial product/ polysaccharide is dextran, and it is produced from bacterium Leuconostoc mesenteroides, with an overall degree of branching of about 5% [1-3][1]. Lim SB, Tingirikari JMR, Seo JS, Li L, Shim S, Seo JH, Han NS. There is a formula that used to calculate the radius (in Angstrom) from the molar mass (in g/mol) of dextran, a=0.33*(MM^0.46) where, a and MM are Macromolecules, 31, 7712-7716. Many works are aimed at determining molecular weights [1, 7, 26], hydrodynamic radius, among other parameters for characterizing the physics and chemistry properties of dextran in solution. Viscotek GmbH, Europe. par | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth | Juil 2, 2022 | mitchell wesley carlson charged | justin strauss net worth The value of kM-H in this work decreases with increasing temperature in contrast to published by reference [34] where kM-H increases. Increased TEWL values are evidence of micropores formation. The molecular mass of dextran as determined by the gel filtration and copper bicinchoninate method was approximately, 800 kDa. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. Terms and Conditions, Chem. This phenomenon is observed in the case of hyperbranched biopolymers evidencing an increase of a with temperature. Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. The characteristic Rg/Rh value for a globular protein is ~0.775, which means that Rg is smaller than Rh. Polysaccharide hydrogels for modified release formulations. Advances in Carbohydrate Analysis, 1, 63-144. European Polymer Journal, 43, 1744-1753. Correspondence to Biodegradable dextran hydrogels crosslinked by stereocomplex formation for the controlled release of pharmaceutical proteins. Viscosity of Liquids. Superior prebiotic and physicochemical properties of novel dextran from Weissella cibaria JAG8 for potential food applications. TdB Labs produce a wide range of FITC-derivatives (490 nm/520 nm) with a mean molecular weight ranging from 4 kDa to 2000 kDa. 2012;87(6):1728. Gel permeation chromatography of dextrans in parenteral solutions: Calibration procedure development and method validation. Alternatives to animal testing: a review. Hindered diffusion of dextran and ficoll in microporous membranes. The lack of data on the uniformity of intrinsic viscosity measurements in the system water/dextran, highlights the significant influence of the solvent and temperature [74]. Masuelli, Martin Alberto (2013). Copyright 2006-2023 Thermo Fisher Scientific Inc. All rights reserved, Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine Fixable, Sulen und Kartuschen fr die Chromatographie, Kunststoffartikel und Zubehr fr das Labor, Spektroskopie, Element- und Isotopenanalyse, Alle Themen fr Hilfe und Support anzeigen, Status und Nachverfolgung von Bestellungen, Reagenzien und Kits fr die Neurowissenschaft, Discover how to track and trace cells by imaging , Hier finden Sie unsere brigen Tracking-Produkte fr Sugetier-Zellkulturen, Molecular Probes Dextran-Konjugate Tabelle14.4. Journal of Physical Chemistry. TEWL measurements were performed before and 30min after microporation. Chem. Unable to load your collection due to an error, Unable to load your delegates due to an error. This conjecture found to be correct. sharing sensitive information, make sure youre on a federal Expert Rev Med Devices. Pharmacol Pharm. Ioan, C.E.